2010.09-2014.06  乐山师范学院化学学院应用化学专业，本科生；

         （导师：成英）

2014.09-2017.06  西南大学化学化工学院分析化学专业，硕士研究生;

         （导师：向云）

2017.09-2021.06  湖南大学化学化工学院化学专业，博士研究生;  

         （导师：聂舟、雷春阳、黄燕）

2021.07-2023.11    乐山师范学院新能源与化学学院，讲师；

2023.12-至今 乐山师范学院新能源与化学学院，副教授；

《Sensors & Actuators: B. Chemical》《Frontiers in Physiology》和《Environmental Science: Water Research & Technology》期刊审稿人

化学生物传感器

1. 基于CRISPR-Cas12a与核酸电路的便携式定量检测新方法在食品检测中的应用研究，国家自然科学基金委员会, 青年科学基金项目

2. 基于DNA分子机器与CRISPR-Cas12a耦合用于肺癌相关miRNAs的精准诊断，四川省科学技术厅，苗子工程

3. 基于CRISPR/Cas12a作为信号扩增技术对癌症标志物的精准诊断，乐山市重点科技项目

一、以第一作者或通讯作者身份发表论文

1. Phosphorothioate-Modified Hairpin G-Triplex Reporter-Assisted Split CRISPR/Cas12a-Powered Biosensor for “Turn-On” Fluorescent Detection of Nucleic Acid and Non-Nucleic Acid Targets, Analytical Chemistry, 2025, 97, 9361-9366.

2. Asymmetric CRISPR-Cas12a powered electrochemical aptasensor for clenbuterol detection based on competitive gRNA mediated cascade signal amplification, Food Chemistry, 2025, 464, 141928.

3. Target-promoted formation of DNA four-way junction for catalytic hairpin assembly amplification and electrochemical SNP assay in folded hairpin structure, Sensors & Actuators: B. Chemical, 2025, 423, 136865.

4. Multiple gRNAs-assisted CRISPR/Cas12a-based portable aptasensor enabling glucometer readout for amplification-free and quantitative detection of malathion, Analytica Chimica Acta, 2025, 1341, 343662.

5. Phosphorothioate-modified G-quadruplex as a signal-on dual-mode reporter for CRISPR/Cas12a-based portable detection of environmental pollutants, Analytica Chimica Acta, 2024, 1308, 342649.

6. Hg²⁺-triggered cascade strand displacement assisted CRISPR-Cas12a for Hg²⁺ quantitative detection using a portable glucose meter, Analytica Chimica Acta, 2023, 1278, 341756.

7. PAM-free cascaded strand displacement coupled with CRISPR-Cas12a for ampliffed electrochemical detection of SARS-CoV-2 RNA, Analytical Biochemistry, 2023, 664, 115046.

8. A CRISPR-Cas autocatalysis-driven feedback amplification network for supersensitive DNA diagnostics, Science Advances, 2021, eabc7802.

9. Amplified and label-free electrochemical detection of a protease biomarker by integrating proteolysis-triggered transcription, Biosensors and Bioelectronics, 2021, 190, 113372.

10. Coupling of proteolysis-triggered transcription and CRISPR-Cas12a for ultrasensitive protease detection, Science China Chemistry, 2021, 64: 330-336.

11. Target-triggered catalytic hairpin assembly and TdT-catalyzed DNA polymerization for amplified electronic detection of thrombin in human serums, Biosensors and Bioelectronics, 2017, 87: 495-500.

12. Cascaded strand displacement for non-enzymatic target recycling amplification and label-free electronic detection of microRNA from tumor cells. Analytica Chimica Acta, 2016, 916: 1-7.

13. DNA-fueled molecular machine enables enzyme-free target recycling amplification for electronic detection of microRNA from cancer cells with highly minimized background noise. Analytical chemistry, 2015, 87(16):8578-8583.

二、授权专利

聂舟，史铠，雷春阳，基于CRISPR-Cas自催化放大网络的核酸检测方法及其应用